|
KMID : 0377619750290040345
|
|
Korean Jungang Medical Journal
1975 Volume.29 No. 4 p.345 ~ p.354
|
|
|
Effects of Prophage Curing of the Recipient Bacteria on Transduction with Staphylococcal Transducing Phage
|
|
Moon Young-Suk
|
|
|
Abstract
|
|
|
Transducing phage 53, yielded by ultraviolet-light induction of phage 53, a lysogenic derivative of Staphylococcus aureus NCTC 8511 (P 53), has been found to be a generalized transduction system in Staphylococcus aureus.
The growth characteristics of phage 53, both temperate and active phage, have been studied. The latend periods in nutrient broth are 48 min in infected sensitive bacterium, and 79 min in ultraviolet-light induced lysogenic bacterium. The burst size, as measured by one-step growth and single-burst experiments, accounts for approximately 28 to 30 phage particles per single lysing cell.
There is little difference in ultraviolet sensitivity between bacteria lysogenic for phage 53 and those non-lysogenic for phage 53. Every ultraviolet-light than for lysogenic bacteria.
Lysogenic recipients are more efficient for non-irradiated transducing phage, in contrast to irradiated transducing phage. It has been shown that ultraviolet-light enhances the efficiency of transducing phage 53.
Curing of prophage has been shown for transductants on the transduction process, only when transducing phage and original prophage which the recipients harbor, have the same lytic host ranges.
In the presence of chloramphenicol, increase in transduction frequency and loss of prophage are not observed.
Defective lysogenic bacteria NCTC 8511 (P 53 de SM r) produced by ultraviolet-light irradiation is not induced by P 53 de SM r determinant to multiply extensively.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|